Biotechnology of plant secondary metabolism [کتاب لاتین]

/ edited by Arthur Germano Fett-Neto, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil

Record Identifier: 167906
Added Entry: Fett-Neto, Arthur Germano,1967-editor
Old Record Identifier: 165159
Document Number: 6
Title and statement of responsibility: Biotechnology of plant secondary metabolism،methods and protocols// edited by Arthur Germano Fett-Neto, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil
Physical Description: xi, 180 pages illustrations (some color) 26 cm
Series Title: (Methods in molecular biology,1064-3745
Series Title: (Springer protocols)
subject: Plant cells and tissues Laboratory manuals
subject: Plants- metabolism
subject: Plants- genetics
subject: Secondary Metabolism
subject: Biotechnology
subject: Laboratory Manuals
subject: Plant cells and tissues.$2 fast$0 (OCoLC)fst01065318
subject: Laboratory manuals.$2 fast$0 (OCoLC)fst01920776
Other Physical Details: illustrations (some color)
Dimensions: 26 cm
General Notes: Language: انگلیسی
Notes Pertaining to Publication, Distribution, Etc: Print
Internal Bibliographies/Indexes Note: ng
Internal Bibliographies/Indexes Note: Includes bibliographical references and index.
Contents Note: This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry.--Source other than Library of Congress. Elicitation approaches for withanolide production in hairy root culture of Withania somnifera (L.) Dunal /Ganeshan Sivanandhan [and others] -- Stimulant paste preparation and bark streak tapping technique for pine oleoresin extraction /Thanise Nogueira Fuller [and others] --Modified protocol for high-quality RNA extraction from oleoresin-producing adult pines /Julio Cesar de Lima [and others] --Collection of apoplastic fluids from Arabidopsis thaliana leaves /Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier --From plant extract to a cDNA encoding a glucosyltransferase candidate : proteomics and transcriptomics as tools to help elucidate saponin biosynthesis in Centella asiatica / Fernanda de Costa [and others] --Production of recombinant caffeine synthase from guarana (Paullinia cupana var. sorbilis) in Escherichia coli / Flavia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera -- Heterologous expression and characterization of mimosinase from Leucaena leucocephala /Vishal Singh Negi and Dulal Borthakur --Production of aromatic plant terpenoids in recombinant baker's yeast /Anita Emmerstorfer-Augustin and Harald Pichler --Purification of a recombinant polyhistidine-tagged glucosyltransferase using immobilized metal-affinity chromatography (IMAC) /Fernanda de Costa [and others] --Western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes /Morten Egevang Jorgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier --Laser capture microdissection : avoiding bias in analysis by selecting just what matters /Marcia R. de Almeida and Martina V. Stromvik --Analytical and fluorimetric methods for the characterization of the transmembrane transport of specialized metabolites in plants /Ines Carqueijeiro [and others] --Protoplast transformation as a plant-transferable transient expression system /Patricia Duarte [and others] --Design, construction, and validation of artificial MicroRNA vectors using agrobacterium-mediated transient expression system /Basdeo Bhagwat [and others] --Knockdown of polyphenol oxidase gene expression in potato (Solanum tuberosum L.) with artificial MicroRNAs /Ming Chi [and others].
Title: Methods in molecular biology (Clifton, N.J.) ;v. 1405.1064-3745
Title: Springer protocols (Series)1949-2448
number(ISBN): 9781493933 1493933914
Number: ‎5‎8‎1‎.‎7‎.‎8‎8‎
Book number: QK
Library of Congress Classification‭‭: QK ‎.‎B‎5‎8‎5‎ ‎7‎2‎5‎ ‎2‎0‎1‎6‎
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499 1 QK‎7‎‎2‎‎5‎‎ ‎‎.‎B‎5‎‎8‎‎5‎‎ ‎‎2‎‎0‎‎1‎‎6‎ 405451
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