| Record Identifier: | 167906 |
| Added Entry: | Fett-Neto, Arthur Germano,1967-editor |
| Old Record Identifier: | 165159 |
| Document Number: | 6 |
| Title and statement of responsibility: | Biotechnology of plant secondary metabolism،methods and protocols// edited by Arthur Germano Fett-Neto, Federal University of Rio Grande do Sul, Porto Alegre, RS, Brazil |
| Physical Description: | xi, 180 pages illustrations (some color) 26 cm |
| Series Title: | (Methods in molecular biology,1064-3745 |
| Series Title: | (Springer protocols) |
| subject: | Plant cells and tissues Laboratory manuals |
| subject: | Plants- metabolism |
| subject: | Plants- genetics |
| subject: | Secondary Metabolism |
| subject: | Biotechnology |
| subject: | Laboratory Manuals |
| subject: | Plant cells and tissues.$2 fast$0 (OCoLC)fst01065318 |
| subject: | Laboratory manuals.$2 fast$0 (OCoLC)fst01920776 |
| Other Physical Details: | illustrations (some color) |
| Dimensions: | 26 cm |
| General Notes: | Language: انگلیسی |
| Notes Pertaining to Publication, Distribution, Etc: | |
| Internal Bibliographies/Indexes Note: | ng |
| Internal Bibliographies/Indexes Note: | Includes bibliographical references and index. |
| Contents Note: | This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry.--Source other than Library of Congress. Elicitation approaches for withanolide production in hairy root culture of Withania somnifera (L.) Dunal /Ganeshan Sivanandhan [and others] -- Stimulant paste preparation and bark streak tapping technique for pine oleoresin extraction /Thanise Nogueira Fuller [and others] --Modified protocol for high-quality RNA extraction from oleoresin-producing adult pines /Julio Cesar de Lima [and others] --Collection of apoplastic fluids from Arabidopsis thaliana leaves /Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier --From plant extract to a cDNA encoding a glucosyltransferase candidate : proteomics and transcriptomics as tools to help elucidate saponin biosynthesis in Centella asiatica / Fernanda de Costa [and others] --Production of recombinant caffeine synthase from guarana (Paullinia cupana var. sorbilis) in Escherichia coli / Flavia Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera -- Heterologous expression and characterization of mimosinase from Leucaena leucocephala /Vishal Singh Negi and Dulal Borthakur --Production of aromatic plant terpenoids in recombinant baker's yeast /Anita Emmerstorfer-Augustin and Harald Pichler --Purification of a recombinant polyhistidine-tagged glucosyltransferase using immobilized metal-affinity chromatography (IMAC) /Fernanda de Costa [and others] --Western blot protocol for detection of proteins heterologously expressed in Xenopus laevis oocytes /Morten Egevang Jorgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier --Laser capture microdissection : avoiding bias in analysis by selecting just what matters /Marcia R. de Almeida and Martina V. Stromvik --Analytical and fluorimetric methods for the characterization of the transmembrane transport of specialized metabolites in plants /Ines Carqueijeiro [and others] --Protoplast transformation as a plant-transferable transient expression system /Patricia Duarte [and others] --Design, construction, and validation of artificial MicroRNA vectors using agrobacterium-mediated transient expression system /Basdeo Bhagwat [and others] --Knockdown of polyphenol oxidase gene expression in potato (Solanum tuberosum L.) with artificial MicroRNAs /Ming Chi [and others]. |
| Title: | Methods in molecular biology (Clifton, N.J.) ;v. 1405.1064-3745 |
| Title: | Springer protocols (Series)1949-2448 |
| number(ISBN): | 9781493933 1493933914 |
| Number: | 581.7.88 |
| Book number: | QK |
| Library of Congress Classification: | QK .B585 725 2016 |
| شماره ثبت | جزء | نسخه | جلد | بخش | قسمت | مرجع | شماره بازیابی | در دست امانت | تاریخ بازگشت | ملاحظات | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 499 | 1 | QK725 .B585 2016 | 405451 |